Analysis and Investigation of the Genomic Map of Gordonia terrae Bacteriophage SweatNTears

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Abstract Summary


Bacteriophage are the viruses that infect bacteria and are the most abundant biological entity on Earth. There are 5,633 phage sequences in GenBank and one third of these were isolated from a single bacterial species, Mycobacterium smegmatis. In contrast, there are only 142 phages isolated using Gordonia species[1]. In using alternative bacterial species for phage isolation, we can, we can increase the understanding of genomic mosaicism in phage. SweatNTears is a novel lytic phage that forms small, clear plaques with turbid halos on a lawn of G. terrae. It has Siphoviridae particle morphology, belongs to cluster CT, and has a broad host range which infects the host bacteria Rhodococcus erythropolysis and G. rubripertincta. Its genome is 45,197 nucleotides in length with a low GC content (59.9 %) compared to that of the host, 63%. The genome encodes 69 putative proteins including 2 orphams, genes with no relation to other sequences in the database and lacks tRNAs genes. Therefore, the completion of gene mapping for SweatNTears will give a complete understanding of the biological functions of how this novel bacteriophage operates in addition to aiding in further research of the field of study as a whole.

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Biomedical Sciences
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